bio 1d software package Search Results


labimage 1d software  (Kapelan Bio Imaging GmbH)
90
Kapelan Bio Imaging GmbH labimage 1d software
Labimage 1d Software, supplied by Kapelan Bio Imaging GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pm36008945-260-21-24?v=Kapelan+Bio+Imaging+GmbH
Average 90 stars, based on 1 article reviews
labimage 1d software - by Bioz Stars, 2026-07
90/100 stars
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1d scan software package version 15.08b  (scanalytics inc)
90
scanalytics inc 1d scan software package version 15.08b
1d Scan Software Package Version 15.08b, supplied by scanalytics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc11203682-217-11-17?v=scanalytics+inc
Average 90 stars, based on 1 article reviews
1d scan software package version 15.08b - by Bioz Stars, 2026-07
90/100 stars
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bioprofil® image analysis software  (Vilber Lourmat)
90
Vilber Lourmat bioprofil® image analysis software
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Bioprofil® Image Analysis Software, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc00152864-309-15-20?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
bioprofil® image analysis software - by Bioz Stars, 2026-07
90/100 stars
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lab image 1d software  (Kapelan Bio Imaging GmbH)
90
Kapelan Bio Imaging GmbH lab image 1d software
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Lab Image 1d Software, supplied by Kapelan Bio Imaging GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc03189810-193-27-31?v=Kapelan+Bio+Imaging+GmbH
Average 90 stars, based on 1 article reviews
lab image 1d software - by Bioz Stars, 2026-07
90/100 stars
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1d pro v12.2 software package  (PHORETIX INTERNATIONAL LIMITED)
90
PHORETIX INTERNATIONAL LIMITED 1d pro v12.2 software package
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
1d Pro V12.2 Software Package, supplied by PHORETIX INTERNATIONAL LIMITED, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pm26296780-29-16-21?v=PHORETIX+INTERNATIONAL+LIMITED
Average 90 stars, based on 1 article reviews
1d pro v12.2 software package - by Bioz Stars, 2026-07
90/100 stars
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labimage 1d 2008 professional software  (Kapelan Bio Imaging GmbH)
90
Kapelan Bio Imaging GmbH labimage 1d 2008 professional software
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Labimage 1d 2008 Professional Software, supplied by Kapelan Bio Imaging GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pm21685248-53-6-11?v=Kapelan+Bio+Imaging+GmbH
Average 90 stars, based on 1 article reviews
labimage 1d 2008 professional software - by Bioz Stars, 2026-07
90/100 stars
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gel analysis software bio-1d  (Vilber Lourmat)
90
Vilber Lourmat gel analysis software bio-1d
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Gel Analysis Software Bio 1d, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc05880409-56-3-10?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
gel analysis software bio-1d - by Bioz Stars, 2026-07
90/100 stars
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bio-1d advanced software  (Montreal Biotech Inc)
90
Montreal Biotech Inc bio-1d advanced software
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Bio 1d Advanced Software, supplied by Montreal Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc03790733-125-27-30?v=Montreal+Biotech+Inc
Average 90 stars, based on 1 article reviews
bio-1d advanced software - by Bioz Stars, 2026-07
90/100 stars
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semi-automated 1d scan software package vision-capt  (Vilber Lourmat)
90
Vilber Lourmat semi-automated 1d scan software package vision-capt
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Semi Automated 1d Scan Software Package Vision Capt, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc09492725-157-9-15?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
semi-automated 1d scan software package vision-capt - by Bioz Stars, 2026-07
90/100 stars
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1d scan software package vision-capt  (Vilber Lourmat)
90
Vilber Lourmat 1d scan software package vision-capt
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
1d Scan Software Package Vision Capt, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc08526609-128-32-37?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
1d scan software package vision-capt - by Bioz Stars, 2026-07
90/100 stars
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gel image software (bio profil bio 1d  (LTF Labortechnik GmbH)
90
LTF Labortechnik GmbH gel image software (bio profil bio 1d
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Gel Image Software (Bio Profil Bio 1d, supplied by LTF Labortechnik GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/pmc04837237-136-5-10?v=LTF+Labortechnik+GmbH
Average 90 stars, based on 1 article reviews
gel image software (bio profil bio 1d - by Bioz Stars, 2026-07
90/100 stars
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bio-profil 1-d software  (Vilber Lourmat)
90
Vilber Lourmat bio-profil 1-d software
Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using <t>BIOPROFIL®</t> image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.
Bio Profil 1 D Software, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bio+1d+software+package/10__1074_slash_jbc__m001235200-69-11-16?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
bio-profil 1-d software - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using BIOPROFIL® image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.

Journal:

Article Title: Histone deacetylase inhibitors stimulate mitochondrial HMG-CoA synthase gene expression via a promoter proximal Sp1 site

doi:

Figure Lengend Snippet: Mitochondrial HMG-CoA synthase, a target gene for HDAC inhibitors: TSA induces mitochondrial HMG-CoA synthase mRNA expression through mitochondrial HMG-CoA synthase promoter acetylation in the chromatin complex. (A) HT-29 and Caco-2 cells were treated with 0.3 µM TSA (+) or DMSO (–) for 24 h. The mitochondrial HMG-CoA synthase mRNA levels were analyzed by northern blot. Levels of 18S rRNA are also shown to correct for gel loading variation. (B) ChIPs were performed using chromatin isolated from HT-29 and CaCo-2 cells either treated with TSA (+) for 24 h or untreated (–). The chromatin preparations were immunoprecipitated with antibodies against acetylated histone H4 (α-AcH4) and analyzed by PCR to detect the HMG-CoA synthase promoter associated to acetylated histones. Aliquots of chromatin were also analyzed before immunoprecipitation (Input). Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using BIOPROFIL® image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. (C) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter. CaCo-2 chromatin, isolated as described above, was immunoprecipitated with increasing amounts of anti-HDAC1 or preimmune serum (IgG). PCRs shown in (B) and (C) were performed with 5 µl of DNA and 30 cycles of amplification were used.

Article Snippet: Relative histone acetylation levels (Fold of acetylation) were determined by quantitation of PCR product using BIOPROFIL® image analysis software, BIO-1D (Vilber Lourmat) and by correction with the input data and represented as the ratio of relative acetylation in the presence versus the absence of TSA treatment. ( C ) ChIP assay of HDAC1 bound to endogenous mitochondrial HMG-CoA synthase promoter.

Techniques: Expressing, Northern Blot, Isolation, Immunoprecipitation, Quantitation Assay, Software, Amplification